Stratalycs provides you with solutions for streamlining NGS data analysis through effective data management, appropriate analytical strategies and visualization.
Sequencing output grows faster than the systems handling it. We build the storage, pipeline and quality-control layer so the analysis stays tractable as volume increases.
We support the complete workflow, from sample library preparation and sequencing through to bioinformatics analysis, with quality checked at every step. Our clinical services run from sequencing to high-end analysis, including drug and protein interaction studies that help clinicians identify targets. Genomics data can also be integrated with other omics and clinical datasets.
What this includes
Library preparation and sequencing
Sample library preparation and sequencing on Illumina platforms, with quality checked before data reaches analysis.
Resequencing, de novo and assembly
Resequencing, de novo sequencing and genome assembly for human, animal, plant, bacterial and host-pathogen studies.
Primary and secondary analysis
Quality control, alignment, variant calling and annotation with parameters documented per run.
Data management
Storage, naming and metadata conventions that keep runs traceable months later.
Analytical strategy
Choosing the right approach for the experiment rather than the most familiar one, with tailored analysis on request.
Visualisation
Interpretable outputs for both bioinformaticians and the wider research team.
Sequencing applications
- RNA-Seq and small RNA-Seq
- DNA-Seq and whole genome sequencing
- Exome and targeted resequencing
- Methyl-Seq
- ChIP-Seq
- Metagenomics
Exome and targeted resequencing
Most disease-causing variants lie in exons, splice sites and promoter regions - around 2% of the human genome - so exome sequencing is a faster, lower-cost route when whole-genome sequencing is not needed.
- Variants detected across coding exons
- Optional content for UTRs and microRNA to study gene regulation
- Libraries prepared in as little as one day
- Around 4-5 Gb of sequencing per exome
RNA-Seq
RNA-Seq measures the abundance and sequence of RNA transcripts with far higher coverage and resolution than microarray or Sanger-based methods, which makes it the basis for accurate differential expression analysis.
- Discovery of novel exons, genes and splice isoforms
- High dynamic range for expression analysis
- Pipelines chosen for your study design, since no single protocol suits every question
- Known limitations such as primer bias and reverse-transcription errors accounted for in analysis
Expertise across organisms
- Human genomics and diagnostics
- Animal genomics
- Plant genomics and agrigenomics
- Bacterial and fungal genomics
- Metagenomics
- Host-pathogen interactions
Sequencing platforms
| Platform | Output | Read type | Typical applications |
|---|---|---|---|
| Illumina MiSeq | 2 × 150 bp | Paired-end | Bacterial, fungal, metagenomics |
| Illumina HiSeq | 250 million reads | Paired-end | Agriculture, clinical, veterinary, plant, human |
| Illumina HiSeq X Ten | 375 million reads | Paired-end | Agriculture, clinical, veterinary, plant, human |
| Illumina NextSeq 500 | 400 million reads | Paired-end | Agriculture, clinical, veterinary, plant, human |
What you get
- One partner from sample to interpreted result
- Analysis that scales as sequencing volume grows
- Runs traceable back to their parameters and inputs
- Quality issues caught before downstream interpretation
- Results readable by non-specialists